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Several years ago, two different publications appeared showing that prolonged incubation of blood cultures beyond the initial five-day protocol for patients with suspected fastidious bacterial endocarditis did not yield significant additional pathogens with today's modern blood-culture media and automated methods.

Blood Culture-negative Endocarditis: What Can the Laboratory Bring to the Table?

Several years ago, two different publications appeared showing that prolonged incubation of blood cultures beyond the initial five-day protocol for patients with suspected fastidious bacterial endocarditis did not yield significant additional pathogens with today's modern blood-culture media and automated methods.